RBS
BCRBS

Part:BBa_M36293:Design

Designed by: Dominique Dabija, Christopher Jackson, Debha Amatya   Group: Stanford BIOE44 - S11   (2011-12-06)

5' Bicistronic UTR, includes ATG start


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

5' UTR based on bicistronic junction architecture. First RBS is strong and drives expression of short leader cistron. Second RBS internal to first cistron control translation of gene of interest. Architecture is optimized so that downstream CDS for gene of interest does not have a chance to form a secondary structure with the 5' UTR. See BIOFAB.org for more information.

Source

Escherichia coli

References

BIOFAB part BD12, apFAB535